Composite

Part:BBa_K101006:Design

Designed by: Ellen Martin   Group: iGEM08_Minnesota   (2008-07-28)


Construct with TetR/p22mnt promoter, RBS, RFP, Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Majority of design considerations were dealt with during the design of the dually repressed promoter (K101000). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.


Source

Construct is composed of: TetR promoter (R0040), p22mnt promoter (R0073), RBS (B0032), RFP (J06504), and Terminator (B1006).

References